A method to obtain axenic Angiostrongylus vasorum first-stage larvae from dog feces.
Parasitol Res
; 89(2): 89-93, 2003 Jan.
Article
em En
| MEDLINE
| ID: mdl-12489005
An improved method to obtain a large number of axenic larvae of Angiostrongylus vasorum from fecal samples was developed in the present study. The procedure here in reported consisted of obtaining larvae using a modified Baermann technique, followed by an additional filtration step. This isolation technique recovered almost 90% of the living larvae in a clean preparation. Isolated larvae were submitted to decontamination treatments with either sodium hypochlorite or antibiotic cocktail solutions. The axenic status, as confirmed by oral inoculation of decontaminated larvae into germ-free mice, was only achieved using larvae treated with 0.5% sodium hypochlorite solution for 10 min. The isolation and decontamination treatment did not affect larval viability. Treated larvae remained viable and infective to the invertebrate host.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças do Cão
/
Fezes
/
Filtração
/
Vida Livre de Germes
/
Angiostrongylus
Limite:
Animals
Idioma:
En
Revista:
Parasitol Res
Assunto da revista:
PARASITOLOGIA
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Alemanha