Effect of albumin and polyvinyl alcohol on the vitality, motility and acrosomal integrity of canine spermatozoa incubated in vitro.
Reprod Domest Anim
; 37(6): 347-51, 2002 Dec.
Article
em En
| MEDLINE
| ID: mdl-12464073
Sperm culture media used for in vitro fertilization (IVF) procedures are important factors concerning the viability, motility and acrosomal integrity of spermatozoa. The aim of this study was to investigate the effects of three different sperm diluting media, tissue culture medium (TCM-199), sperm culture medium (Sp-TALP) and human tubular fluid (HTF) supplemented with varying concentrations of bovine serum albumin (1, 4 and 6%) or polyvinyl alcohol (0.8%) on the acrosomal integrity, motility and viability of canine spermatozoa. Ejaculates collected from four dogs were diluted in all media and spermatozoa were separated from seminal plasma by the swim-up technique. Sperm progressive motility was assessed using a phase contrast microscope. Viability and acrosomal integrity were evaluated using a dual stain technique (Giemsa-Trypan blue). The results demonstrated that the number of live canine spermatozoa was similar in culture media supplemented or not supplemented with macromolecules. A minimal concentration of albumin (1%) in the three media showed similar effects on vitality, motility and acrosomal integrity, as had higher concentrations (4 and 6%). The percentage of acrosome-intact spermatozoa was markedly higher after HTF (94.1%) than after TCM-199 (70.1%) or Sp-TALP (71.0%) without supplementation. It is concluded that serum bovine albumin, irrespective of the concentration, preserved sperm viability and function, and HTF is the most suitable medium for preserving the acrosome in canine spermatozoa prepared for in vitro manipulation through short incubation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Álcool de Polivinil
/
Capacitação Espermática
/
Motilidade dos Espermatozoides
/
Albumina Sérica
/
Reação Acrossômica
/
Meios de Cultura
/
Cães
Limite:
Animals
Idioma:
En
Revista:
Reprod Domest Anim
Assunto da revista:
MEDICINA REPRODUTIVA
/
MEDICINA VETERINARIA
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Chile
País de publicação:
Alemanha