Expression and activity of matrix metalloproteinase-2 (MMP-2) in the development of rat first molar tooth germ.
Braz Dent J
; 13(2): 97-102, 2002.
Article
em En
| MEDLINE
| ID: mdl-12238810
Tooth germ development is associated with morphological and biochemical changes of the dental papilla and enamel organ. Enzymes with gelatinolytic activities were studied by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and enzymography in tooth germ of newborn to 15-day-old rats. Three major bands with gelatinolytic activity were detected at all periods and characterized as the latent and active forms of MMP-2 using their molecular weight and activity dependent on Zn++ and Ca++ ions as criteria. Expression and activity of MMP-2 increased progressively from 0 to 15 days after birth. Mechanical separation of the tooth germ from 10-day-old rats showed that the gelatinolytic activity was localized mainly in the dental papilla and not the dental organ. These data indicate that the expression and activity of MMP-2 varies during the development and maturation of rat first molar tooth germ.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Germe de Dente
/
Metaloproteinase 2 da Matriz
/
Dente Molar
Limite:
Animals
Idioma:
En
Revista:
Braz Dent J
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Brasil