Pregnancy rates after transfer of frozen bovine embryos: a field trial.
Theriogenology
; 58(5): 963-72, 2002 Sep.
Article
em En
| MEDLINE
| ID: mdl-12212895
An efficient and practical technique for bovine embryo cryopreservation is a fundamental issue in the widespread use of embryo transfer. The present study shows results obtained in field experiments. In the first experiment, two slow-freezing methods using glycerol and a one-step method using ethylene glycol were compared: glycerol added in two steps (5 and then 10%), glycerol added in one step (10%) and 1.5 M ethylene glycol with direct transfer. The three methods were equally effective; pregnancy rates of 40.4, 39.1 and 45.4%, respectively were achieved. In the second experiment, using 1.5 M ethylene glycol with direct transfer, 20 and 5 min of equilibration of the cryoprotectant were tested. There were no observed significant differences in pregnancy rates. In the third experiment, ethylene glycol and propylene glycol were combined with three sucrose concentrations (0, 0.1 or 0.3 M) in a one-step method. It was observed that ethylene glycol and 0.1 M sucrose yielded the highest pregnancy rate, not differing from fresh controls. Similar pregnancy rates were noted after using multiple-step or one-step methods, but the one-step method is preferable due to its simplicity and applicability to field conditions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bovinos
/
Criopreservação
/
Transferência Embrionária
Limite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Theriogenology
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Estados Unidos