Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats.
Neurosci Lett
; 329(3): 344-8, 2002 Sep 06.
Article
em En
| MEDLINE
| ID: mdl-12183045
Mineralocorticoids (MC) play an important role in development of salt appetite. Part of this effect involves the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei, in which MC treatment increases arginine vasopressin (AVP) synthesis and release. Since the AVP system is also modulated by nitric oxide (NO), we studied if deoxycorticosterone acetate (DOCA) treatment changed the number of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) active neurons and neuronal NO synthase (nNOS)-immunoreactive (IR) cells in the PVN and SON. After four injections of DOCA (10 mg/rat per day), rats developed a salt appetite and increased NADPH-d active and nNOS-IR neurons in both nuclei. A single DOCA injection did not change salt consumption or nNOS-IR cells, but increased the number of NADPH-d positive neurons in the PVN only. Therefore, while acute MC treatment stimulated the activity of pre-existing enzyme, chronic steroid treatment recruited additional neurons showing nNOS immunoreactivity/NADPH-d activity. These data suggest a role for NO produced in the PVN and SON in DOCA stimulatory effects on AVP mRNA and salt appetite.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Núcleo Hipotalâmico Paraventricular
/
Hipotálamo Anterior
/
Óxido Nítrico Sintase
/
Desoxicorticosterona
/
NADPH Desidrogenase
Limite:
Animals
Idioma:
En
Revista:
Neurosci Lett
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Irlanda