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Association of severe noncerebral Plasmodium falciparum malaria in Brazil with expressed PfEMP1 DBL1 alpha sequences lacking cysteine residues.
Kirchgatter, Karin; Portillo, Hernando del A.
Afiliação
  • Kirchgatter K; Departmento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Brazil.
Mol Med ; 8(1): 16-23, 2002 Jan.
Article em En | MEDLINE | ID: mdl-11984002
BACKGROUND: Cytoadherence and rosetting contribute to the development of severe Plasmodium falciparum malaria. In Brazil,severe falciparum malaria is mostly associated with renal or pulmonary complications and very rarely with cerebral malaria. The most N-terminal DBL1 alpha domain of PfEMP1, a protein encoded by the var multigene family mediates rosetting. We analyzed parasites of Brazilian patients with severe malaria to determine whether there were particular DBL1 alpha var sequences predominantly expressed in such patients. MATERIALS AND METHODS: DBL1 alpha var sequences were obtained from parasites of Brazilian patients with severe and mild malaria and were analyzed by standard bioinformatic programs. Three hundred twenty var DBL1 alpha sequences obtained from 80 Brazilian patients with mild malaria were spotted in high-density filters and hybridized to probes representing predominantly expressed sequences in parasites from patients with severe malaria. A DBL1 alpha domain was expressed in bacteria and used to demonstrate its binding capacity to erythrocytes by immunofluorescence. RESULTS: Forty-three different and unreported DBL1 alpha amino acid sequences were obtained. Sequences predominantly expressed in patients with severe malaria could be subgrouped due to deletions of 1-2-cysteine residues. These sequences were commonly found in the var gene repertoire of parasites from patients with mild malaria, yet they were rarely expressed in these patients. A recombinant protein representing the most abundantly expressed sequence detected in one patient with severe malaria bound directly to uninfected erythrocytes. CONCLUSIONS: This is the first report showing an association of severe noncerebral malaria from Brazil with particular DBL1 alpha sequences.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plasmodium falciparum / Proteínas de Protozoários / Malária Falciparum Tipo de estudo: Risk_factors_studies País/Região como assunto: America do sul / Brasil Idioma: En Revista: Mol Med Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2002 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plasmodium falciparum / Proteínas de Protozoários / Malária Falciparum Tipo de estudo: Risk_factors_studies País/Região como assunto: America do sul / Brasil Idioma: En Revista: Mol Med Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2002 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido