Expression and preliminary X-ray diffraction studies of cytosolic Cu,Zn superoxide dismutase from Schistosoma mansoni.
Acta Crystallogr D Biol Crystallogr
; 57(Pt 12): 1877-80, 2001 Dec.
Article
em En
| MEDLINE
| ID: mdl-11717503
Active cytosolic (CT) Cu,Zn superoxide dismutase from Schistosoma mansoni (SmCTSOD) was recovered after thrombin cleavage of a glutathione-S-transferase linked fusion protein (GST-SmCTSOD) expressed in the presence of the active-site metals. Crystals have been obtained in two space groups, P2(1)2(1)2(1) and P2(1). The former have unit-cell parameters a = 74.64, b = 78.24, c = 95.18 A and typically diffract to 2.2 A. The monoclinic crystals have unit-cell parameters a = 39.27, b = 95.08, c = 78.41 A, beta = 103.55 degrees and diffract to at least 1.55 A. The calculated solvent content of the crystals is compatible with two dimers of SmCTSOD in the asymmetric unit in both cases. Molecular-replacement solutions have been obtained for both crystal forms and show that slight distortions in the crystal packing relate one form to the other.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Schistosoma mansoni
/
Superóxido Dismutase
Limite:
Animals
Idioma:
En
Revista:
Acta Crystallogr D Biol Crystallogr
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Estados Unidos