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Preliminary analysis of Sm14 in distinct fractions of Schistosoma mansoni adult worm extract.
Thaumaturgo, N; Vilar, M M; Diogo, C M; Edelenyi, R; Tendler, M.
Afiliação
  • Thaumaturgo N; Laboratório de Esquistossomose Experimental, Departamento de Helmintologia, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, 21045-900, Brasil.
Mem Inst Oswaldo Cruz ; 96 Suppl: 79-83, 2001.
Article em En | MEDLINE | ID: mdl-11586430
In previous studies it was shown that the recombinant molecule, r-Sm14, induces high levels of protection against Schistosoma mansoni infection in two outbred animal models and immune crossprotection against infection by Fasciola hepatica in Swiss outbred mice. r-Sm14 was derived from a living worm extract, called SE, and is being developed as the molecular basis of an anti-helminth bivalent vaccine against the two parasites, for medical and veterinary application. Present data refer to SDS-PAGE and Western Blotting analysis of four different preparations of S. mansoni adult worms focusing Sm14 identification. The extracts correspond to the initial fraction of the SE extraction process, containing products released by living worms (SEi); SE2, reextraction of adult worms in PBS; and SE of separated male and female adult worms. In all extracts it was possible to detect the component of 14 kDa, that was recognized by specific anti-rSm14 antibody raised in rabbits.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Membrana Transportadoras / Schistosoma mansoni / Esquistossomose mansoni / Proteínas de Helminto / Proteínas de Neoplasias / Proteínas do Tecido Nervoso Limite: Animals Idioma: En Revista: Mem Inst Oswaldo Cruz Ano de publicação: 2001 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Membrana Transportadoras / Schistosoma mansoni / Esquistossomose mansoni / Proteínas de Helminto / Proteínas de Neoplasias / Proteínas do Tecido Nervoso Limite: Animals Idioma: En Revista: Mem Inst Oswaldo Cruz Ano de publicação: 2001 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil