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Slotoxin, alphaKTx1.11, a new scorpion peptide blocker of MaxiK channels that differentiates between alpha and alpha+beta (beta1 or beta4) complexes.
Garcia-Valdes, J; Zamudio, F Z; Toro, L; Possani, L D; Possan, L D.
Afiliação
  • Garcia-Valdes J; Department of Molecular Recognition and Structural Biology, Institute of Biotechnology, National Autonomous University of Mexico, Cuernavaca, Mexico.
FEBS Lett ; 505(3): 369-73, 2001 Sep 21.
Article em En | MEDLINE | ID: mdl-11576530
A novel peptide from Centruroides noxius Hoffmann scorpion venom was isolated and sequenced. The 37 amino acid peptide belongs to the charybdotoxin sub-family (alphaKTx1) and was numbered member 11. alphaKTx1.11 has 75% sequence identity with iberiotoxin and 54% with charybdotoxin. alphaKTx1.11 revealed specificity for mammalian MaxiK channels (hSlo), thus, was named slotoxin. Slotoxin blocks the MaxiK pore-forming alpha subunit reversibly (K(d)=1.5 nM). Slotoxin association with alpha+beta (beta1 or beta4) channels was approximately 10 times slower than iberiotoxin and charybdotoxin, leading to a lack of effect on alpha+beta4 when tested at 100 nM for 5 min. Thus, slotoxin is a better tool to distinguish MaxiK alpha+beta complexes.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Venenos de Escorpião / Canais de Potássio Cálcio-Ativados / Bloqueadores dos Canais de Potássio Idioma: En Revista: FEBS Lett Ano de publicação: 2001 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido
Buscar no Google
Adicionar na Minha BVS
Imprimir
XML
PubMed Links

Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Venenos de Escorpião / Canais de Potássio Cálcio-Ativados / Bloqueadores dos Canais de Potássio Idioma: En Revista: FEBS Lett Ano de publicação: 2001 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido