Glycol methacrylate: an alternative method for embedding subcutaneous implants.
J Endod
; 27(4): 266-8, 2001 Apr.
Article
em En
| MEDLINE
| ID: mdl-11485264
The aim of this investigation was to study the use of glycol methacrylate (GMA) as an embedding material for rat subcutaneous tissue, which received implants of tubes with endodontic sealer. After fixation, the specimens were dehydrated in a growing alcohol series up to 95%, immersed in infiltration GMA solution and then in embedding solution. The blocks were cut into 3.0 microm sections and stained with hematoxylin-eosin. The quality of cell definition and staining allowed a quantitative analysis of the cells infiltrated in the end of the tubes. It was also possible to identify each type of inflammatory cell. Moreover, it was possible to distinguish clearly chronic from acute inflammatory cells. The GMA technique is easy to execute and reproducible, and provides a better definition of tissue cells, thus permitting definition of the degree of the inflammatory process. Therefore, it is an excellent alternative for the evaluation of the biocompatibility of endodontic sealers.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Materiais Restauradores do Canal Radicular
/
Pele
/
Inclusão do Tecido
/
Metacrilatos
Limite:
Animals
Idioma:
En
Revista:
J Endod
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Estados Unidos