DL-Buthionine-S,R-sulfoximine affects intestinal alkaline phosphatase activity.
Comp Biochem Physiol C Toxicol Pharmacol
; 129(2): 85-91, 2001 Jun.
Article
em En
| MEDLINE
| ID: mdl-11423381
The susceptibility of intestinal alkaline phosphatase to DL-buthionine-S,R-sulfoximine was investigated in chicks fed a commercial diet. The results show that DL-buthionine-S,R-sulfoximine produced inhibition of intestinal alkaline phosphatase activity. This effect showed dose- and time-dependency and it was caused by either in vivo DL-buthionine-S,R- sulfoximine administration or in vitro DL-buthionine-S,R-sulfoximine incubation with villus tip enterocytes. DL-Buthionine-S,R-sulfoximine did not act directly on intestinal alkaline phosphatase but it provoked glutathione depletion which led to changes in the redox state of the enterocyte as shown by the production of free hydroxyl radicals and an incremental increase in the carbonyl content of proteins. The reversibility of the buthionine sulfoximine effect on intestinal alkaline phosphatase was proved by addition of glutathione monoester to the duodenal loop.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Galinhas
/
Cálcio
/
Butionina Sulfoximina
/
Fosfatase Alcalina
/
Mucosa Intestinal
Limite:
Animals
Idioma:
En
Revista:
Comp Biochem Physiol C Toxicol Pharmacol
Assunto da revista:
FARMACOLOGIA
/
TOXICOLOGIA
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Estados Unidos