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Human phosphoglucose isomerase: expression, purification, crystallization and preliminary crystallographic analysis.
Cordeiro, A T; Godoi, P H; Delboni, L F; Oliva, G; Thiemann, O H.
Afiliação
  • Cordeiro AT; Laboratory of Protein Crystallography and Structural Biology, Physics Institute of São Carlos, University of São Paulo - USP, Av. Trabalhador Sãocarlense 400, PO Box 369, 13566-590 São Carlos - SP, Brazil.
Acta Crystallogr D Biol Crystallogr ; 57(Pt 4): 592-5, 2001 Apr.
Article em En | MEDLINE | ID: mdl-11264592
Phosphoglucose isomerase (PGI) is the second enzyme in the glycolytic pathway and catalyzes an aldose-ketose isomerization. Outside the cell, PGI has been found to function as both a cytokine and as a growth factor. The human pgi gene was cloned and the expressed enzyme was purified to homogeneity. Isomorphous crystals were obtained under two conditions and belong to the P2(1)2(1)2(1) space group, with unit-cell parameters a = 80.37, b = 107.54, c = 270.33 A. A 94.7% complete data set was obtained and processed to a limiting resolution of 2.6 A. The asymmetric unit contains two hPGI dimers according to density calculations, a self-rotation function map and molecular-replacement solution.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glucose-6-Fosfato Isomerase Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Acta Crystallogr D Biol Crystallogr Ano de publicação: 2001 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Buscar no Google
Adicionar na Minha BVS
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PubMed Links

Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glucose-6-Fosfato Isomerase Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Acta Crystallogr D Biol Crystallogr Ano de publicação: 2001 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos