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Differences between coding and non-coding regions in the Trichomonas vaginalis genome: an actin gene as a locus model(1).
Espinosa, N; Hernández, R; López-Griego, L; Arroyo, R; López-Villaseñor, I.
Afiliação
  • Espinosa N; Departamento de Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado postal 70-228, CP 04510, México D.F., Mexico.
Acta Trop ; 78(2): 147-54, 2001 Feb 23.
Article em En | MEDLINE | ID: mdl-11230824
The sequence of a cloned genomic fragment of Trichomonas vaginalis containing a complete actin gene was determined. An uninterrupted open reading frame of 1128 nucleotides was found that codes for an actin gene. Two overlapped consensus promoter sequences for T. vaginalis were found 12 nucleotides upstream the actin initiation codon. In addition to actin, two incomplete open reading frames were found at the 5' and 3' ends of the clone. These two sequences are expressed and showed similarity to adenylate cyclase genes and a yeast hypothetical protein. The overall sequence showed a higher G+C content and a lower frequency of repeated sequences in the coding regions when compared with the non-coding regions. A similar unequal nucleotide distribution was found in various T. vaginalis genes retrieved from data bases.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Trichomonas vaginalis / Actinas Limite: Animals Idioma: En Revista: Acta Trop Ano de publicação: 2001 Tipo de documento: Article País de afiliação: México País de publicação: Holanda
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Trichomonas vaginalis / Actinas Limite: Animals Idioma: En Revista: Acta Trop Ano de publicação: 2001 Tipo de documento: Article País de afiliação: México País de publicação: Holanda