Platelet Na+, K+-ATPase activity as a possible peripheral marker for the neurotoxic effects of phenylalanine in phenylketonuria.

Bedin, M; Estrella, C H; Duarte, D V; Ponzi, D; Dutra-Filho, C S; Wyse, A T; Wajner, M; Wannmacher, C M

Bedin, M; Estrella, C H; Duarte, D V; Ponzi, D; Dutra-Filho, C S; Wyse, A T; Wajner, M; Wannmacher, C M.

Afiliação

Bedin M; Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.

Metab Brain Dis ; 15(2): 115-21, 2000 Jun.

Article em En | MEDLINE | ID: mdl-11092578

RESUMO

The in vitro effects of phenylalanine or alanine alone or combined on Na+, K+-ATPase activity in membranes from human platelets were investigated. The enzyme activity was assayed in membranes prepared from platelet-rich plasma of healthy donors. Phenylalanine or alanine were added to the assay to final concentrations of 0.3 to 1.2 mM, similar to those found in plasma of phenylketonuric patients. Phenylalanine inhibited Na+, K+-ATPase activity by 20-50% [F(4,25)=11.47 ; p<0.001]. Alanine had no effect on Na+, K+-ATPase activity but when combined with phenylalanine prevented the enzyme inhibition. These results, allied to others previously reported on brain Na+, K+-ATPase activity, may reflect a general inhibitory effect of phenylalanine on this important enzyme activity. Therefore, it is possible that measurement of Na+, K+-ATPase activity in platelets from PKU patients may be a useful peripheral marker for the neurotoxic effects of phenylalanine.

Assuntos

Alanina/toxicidade; Plaquetas/efeitos dos fármacos; Neurotoxinas/toxicidade; Fenilalanina/toxicidade; Fenilcetonúrias/enzimologia; Fenilcetonúrias/fisiopatologia; ATPase Trocadora de Sódio-Potássio/efeitos dos fármacos; Biomarcadores/sangue; Plaquetas/citologia; Plaquetas/enzimologia; ATPase de Ca(2+) e Mg(2+)/efeitos dos fármacos; ATPase de Ca(2+) e Mg(2+)/metabolismo; Membrana Celular/efeitos dos fármacos; Membrana Celular/enzimologia; Humanos; ATPase Trocadora de Sódio-Potássio/metabolismo

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fenilalanina / Fenilcetonúrias / Plaquetas / ATPase Trocadora de Sódio-Potássio / Alanina / Neurotoxinas Limite: Humans Idioma: En Revista: Metab Brain Dis Assunto da revista: CEREBRO / METABOLISMO Ano de publicação: 2000 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

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