Purification and partial characterization of a chromate reductase from Bacillus.
Rev Latinoam Microbiol
; 39(1-2): 73-81, 1997.
Article
em En
| MEDLINE
| ID: mdl-10932716
A soluble NADH-dependent enzyme capable of reducing hexavalent chromium [Cr(VI)] to the trivalent form [Cr(III)] was purified from chromate-resistant Bacillus QC1-2. An enriched single protein band of 24 kDa was observed by SDS-PAGE following HPLC ion-exchange and size-exclusion procedures. In the latter step, the chromate reductase showed a molecular mass of 44 kDa, which suggested that the enzyme consists of two subunits of about 24 kDa. Purified chromate reductase displayed optimal activity at a temperature and pH of 37 degrees C and 7.0, respectively. The enzyme showed a Km of 0.35 mM for chromate and a Vmax of 50 nmol Cr(VI) reduced per minute per mg protein.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxirredutases
/
Bacillus
/
Proteínas de Bactérias
Idioma:
En
Revista:
Rev Latinoam Microbiol
Ano de publicação:
1997
Tipo de documento:
Article
País de publicação:
México