A priori estimation of accuracy and of the number of wells to be employed in limiting dilution assays.
Braz J Med Biol Res
; 33(8): 937-47, 2000 Aug.
Article
em En
| MEDLINE
| ID: mdl-10920436
The use of limiting dilution assay (LDA) for assessing the frequency of responders in a cell population is a method extensively used by immunologists. A series of studies addressing the statistical method of choice in an LDA have been published. However, none of these studies has addressed the point of how many wells should be employed in a given assay. The objective of this study was to demonstrate how a researcher can predict the number of wells that should be employed in order to obtain results with a given accuracy, and, therefore, to help in choosing a better experimental design to fulfill one's expectations. We present the rationale underlying the expected relative error computation based on simple binomial distributions. A series of simulated in machina experiments were performed to test the validity of the a priori computation of expected errors, thus confirming the predictions. The step-by-step procedure of the relative error estimation is given. We also discuss the constraints under which an LDA must be performed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Computação Matemática
/
Técnicas de Diluição do Indicador
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Braz J Med Biol Res
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Brasil