Comparative analysis of three methods to assess viability of mammalian cells in culture.
Biocell
; 23(1): 65-72, 1999 Apr.
Article
em En
| MEDLINE
| ID: mdl-10904534
Release of lactate dehydrogenase (LDH) from the cytoplasmic compartment, trypan blue exclusion and methylthiazole tetrazolium (MTT) colorimetric assays were compared with regard to their sensitivity in detecting damage of human cultured epithelial cells induced by sodium fluoride or puromycin. LDH assay did not detect any difference between controls and cells treated with either of the two drugs. Cell monolayers treated with 0.3% sodium fluoride or 10(-2) M puromycin presented higher percentages of cells that took up the trypan blue dye than controls but monolayers treated with lower drug concentrations did not differ from controls. Viability measured by MTT assay was the most sensitive assay, detecting a dose-dependent impairment of cell function after treatment with the two drugs. Moreover, MTT offered major advantages in speed, simplicity and precise quantitation over the other viability assays.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sobrevivência Celular
/
Técnicas de Cultura de Células
/
Fígado
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biocell
Assunto da revista:
BIOLOGIA MOLECULAR
/
DIAGNOSTICO POR IMAGEM
/
HISTOCITOQUIMICA
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Estados Unidos