Effect of magnesium ions on fermentative and respirative functions in Pichia stipitis under oxygen-restricted growth.
Microbiol Res
; 155(1): 31-5, 2000 Apr.
Article
em En
| MEDLINE
| ID: mdl-10830897
Mg2+ level affected growth, xylitol and ethanol production by P. stipitis grown under microaerophilic conditions. Low Mg2+ level (1 mM) directed the C flux from ethanol to xylitol, with no effect on xylose consumption rate. The addition of pyrazole, an alcohol dehydrogenase (ADH) inhibitor, had the same effect, even in conditions of Mg2+ excess (4 mM), indicating a negative interaction between ADH and Mg2+ ions (p << 0.01). Cells grown either with pyrazole or Mg limitation increased their intracellular NADH concentration about 3 times, but displayed no significant differences in ADH specific activities (1,000 U/mg protein, +/- 10%). In contrast, no interaction was measured between Mg and antimycin A, excluding the possibility that Mg2+ limitation interferes with respiration.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Consumo de Oxigênio
/
Pichia
/
Magnésio
Idioma:
En
Revista:
Microbiol Res
Assunto da revista:
MICROBIOLOGIA
/
SAUDE AMBIENTAL
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Alemanha