Sequence analysis of the rDNA internal transcribed spacer 2 of five species of South American human malaria mosquitoes.
DNA Seq
; 8(4): 215-21, 1998 Mar.
Article
em En
| MEDLINE
| ID: mdl-10520449
The rDNA internal transcribed spacer 2 (ITS2) was sequenced for 5 species of mosquitoes that may be important vectors of human malaria in certain regions of South America and are difficult to distinguish by morphology: Anopheles evansae, An. nuneztovari, An. rangeli, An. strodei and An. trinkae. ITS2 sequences from samples collected in Ecuador, Bolivia, Venezuela and Brazil were aligned and compared in order to determine the usefulness of this spacer for the elaboration of species specific primers and DNA probes. The ITS2 was found to be different in size (ranging from 333 to 397 bp) and sequence between all pairs of species. Highly variable regions were found primarily at the 3' end of the spacer and were interspersed with relatively conserved sites. Instraspecific sequence variation was limited to a single transversion between specimens of An. rangeli from distant geographic locations suggesting concerted evolution and homogenization of the ITS2.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Ribossômico
/
RNA Ribossômico 5,8S
/
RNA Ribossômico 28S
/
Anopheles
Limite:
Animals
País/Região como assunto:
America do sul
Idioma:
En
Revista:
DNA Seq
Assunto da revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Estados Unidos
País de publicação:
Reino Unido