Humoral and cell-mediated immune responses in humans to the NSP4 enterotoxin of rotavirus.
J Med Virol
; 59(3): 369-77, 1999 Nov.
Article
em En
| MEDLINE
| ID: mdl-10502271
Rotavirus nonstructural protein NSP4 has recently been suggested to function as a viral enterotoxin and play a role in the pathophysiological mechanism whereby rotaviruses induce diarrhea. The ability of rotavirus NSP4 to stimulate a humoral immune response was examined in naturally infected children and adults, rotavirus vaccinated children, as well as a cellular immune response in adults. In this study, 10 of 10 naturally infected children and 9 of 10 rotavirus-vaccinated children showed a weak humoral IgG immune response to recombinant NSP4 (rNSP4) and/or a synthetic peptide corresponding to residues 114-134 of NSP4. Modest serum IgG antibody responses were detected in 20 of 20 adults. A cellular immune response to rNSP4 and/or NSP4(114-134) were detected in 8 of 10 adults measured either as a T-cell proliferative response (7 of 10), an increased production of IL-2 (6 of 10), or increased production of interferon-gamma (8 of 10). These results indicate that NSP4 induces a humoral immune response in humans and show for the first time that NSP4 stimulates a cellular immune response, possibly including cytolytic T-cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por Rotavirus
/
RNA Polimerases Dirigidas por DNA
/
Proteínas não Estruturais Virais
/
Rotavirus
Limite:
Adult
/
Humans
/
Infant
País/Região como assunto:
America central
/
Europa
/
Nicaragua
Idioma:
En
Revista:
J Med Virol
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Suécia
País de publicação:
Estados Unidos