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Protection against toxoplasmosis in mice immunized with different antigens of Toxoplasma gondii incorporated into liposomes.
Elsaid, M M; Vitor, R W; Frézard, F J; Martins, M S.
Afiliação
  • Elsaid MM; Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brasil.
Mem Inst Oswaldo Cruz ; 94(4): 485-90, 1999.
Article em En | MEDLINE | ID: mdl-10446006
Different toxoplasma antigens were entrapped within liposomes and evaluated, in this form, for their ability to protect Swiss mice against toxoplasma infection: soluble tachyzoite antigen (L/TAg), tissue cyst (L/CAg), tachyzoite plus tissue cyst (L/TCAg) or purified antigen of tachyzoite (L/pTAg). The protein used in L/pTAg was purified from tachyzoites using a stage-specific monoclonal antibody which reacted at a molecular weight of 32 kD in SDS PAGE and silver stain using reduced condition. To compare the immuno-adjuvant action of liposomes and of Freund's Complete Adjuvant (FCA), another group of mice was immunized with soluble tachyzoite antigen (STAg) emulsified in FCA (FCA/TAg). Control groups were inoculated with (STAg) alone, phosphate-buffered saline (PBS), FCA with PBS (FCA/PBS) and empty liposomes (L/PBS). Mice were inoculated subcutaneously with these antigens six, four and two weeks before a challenge with 80 tissue cysts of the P strain of Toxoplasma gondii orally. All mice immunized with or without adjuvant showed a humoral response, as measured by Elisa. However, no correlation was found between antibody titer and protection against the challenge. All mice immunized with L/pTAg or L/TCAg survived (100), whereas 80% and 90% of mice from groups which received respectively PBS or FCA/PBS and L/PBS died. All mice immunized with antigens entrapped within liposomes (L/TAg, L/CAg, L/TCAg and L/pTAg) showed low numbers of intracerebral cysts.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Toxoplasma / Proteínas de Protozoários / Toxoplasmose / Vacinas Protozoárias / Antígenos de Protozoários Limite: Animals Idioma: En Revista: Mem Inst Oswaldo Cruz Ano de publicação: 1999 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Toxoplasma / Proteínas de Protozoários / Toxoplasmose / Vacinas Protozoárias / Antígenos de Protozoários Limite: Animals Idioma: En Revista: Mem Inst Oswaldo Cruz Ano de publicação: 1999 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil