Comparison of a commercial enzyme immunoassay with plaque reduction neutralization for maternal and infant measles antibody measurement.
Rev Inst Med Trop Sao Paulo
; 41(1): 21-6, 1999.
Article
em En
| MEDLINE
| ID: mdl-10436666
The most practicable assay for measurement of measles IgG (mIgG) in large numbers of sera is an enzyme immunoassay (EIA). To assess how EIA results would agree with those by the gold standard method of plaque reduction neutralization (PRN) we compared the results from the two methods in 43 pairs of maternal and umbilical cord sera, and sera from the corresponding infants when aged 11-14 months. In maternal-cord sera, the differences between mean antibody levels by EIA or PRN were not statistically significant, though in individual sera, differences could be large. However, agreement was less good for infants sera, in which levels of mIgG were very low. The conclusions of a study of transplacental transport of mIgG would not be affected by the use of either technique. When studying waning immunity in infants, PRN should be the method of choice, while results from studies using EIA should be interpreted with caution.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Testes de Neutralização
/
Técnicas Imunoenzimáticas
/
Imunidade Materno-Adquirida
/
Sarampo
/
Vírus do Sarampo
/
Anticorpos Antivirais
Limite:
Female
/
Humans
/
Infant
Idioma:
En
Revista:
Rev Inst Med Trop Sao Paulo
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Portugal
País de publicação:
Brasil