Detection of BHV-1 in a naturally infected bovine fetus by a nested PCR assay.
Vet Res Commun
; 23(2): 133-41, 1999 Mar.
Article
em En
| MEDLINE
| ID: mdl-10359157
Bovine herpesvirus 1 (BHV-1) is frequently associated with abortion in naturally and experimentally infected cattle. Most of the virus isolation and immunofluorescent antibody protocols described in the literature for detecting BHV-1 in bovine foetuses are rather laborious, costly and time-consuming. The detection is described of BHV-1 in the tissues of a naturally aborted bovine foetus by a nested PCR assay with no further hybridization procedures. Optimal results were achieved by filtering the foetal tissues on a chromatography column before DNA extraction, by using two pairs of primers in a nested PCR and by evaluating the amplification products on silver-stained polyacrylamide gels. This nested PCR was faster and easier to perform than the virus isolation test. To our knowledge, this is the first time that BHV-1 has been detected in the tissues of a naturally infected bovine foetus by means of a nested PCR. The test seems to be a practical alternative for rapid detection of BHV-1 in bovine foetus.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças dos Bovinos
/
Reação em Cadeia da Polimerase
/
Infecções por Herpesviridae
/
Herpesvirus Bovino 1
/
Aborto Animal
Tipo de estudo:
Diagnostic_studies
/
Guideline
Limite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Vet Res Commun
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Suíça