First synthesis of a fully active spin-labeled peptide hormone.
FEBS Lett
; 446(1): 45-8, 1999 Mar 05.
Article
em En
| MEDLINE
| ID: mdl-10100612
For the first time in the electron spin resonance (ESR) and peptide synthesis fields, a fully active spin-labeled peptide hormone was reported. The ESR spectra of this alpha-melanocyte stimulating hormone (alpha-MSH) analogue (acetyl-Toac0-alpha-MSH) where Toac is the paramagnetic amino acid probe 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid, suggested a pH-independent conformation and a more restricted movement comparatively to the free Toac. Owing to its equivalent biological potency in a skin pigmentation assay as compared to the native alpha-MSH and its unique characteristic (paramagnetic, naturally fluorescent and fully active), this analogue is of great potential for investigation of relevant physiological roles reported for alpha-MSH.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Alfa-MSH
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Reino Unido