First synthesis of a fully active spin-labeled peptide hormone.

Barbosa, S R; Cilli, E M; Lamy-Freund, M T; Castrucci, A M; Nakaie, C R

Barbosa, S R; Cilli, E M; Lamy-Freund, M T; Castrucci, A M; Nakaie, C R.

Afiliação

Barbosa SR; Departamento de Biofisica, Universidade Federal de São Paulo, SP, Brazil.

FEBS Lett ; 446(1): 45-8, 1999 Mar 05.

Article em En | MEDLINE | ID: mdl-10100612

RESUMO

For the first time in the electron spin resonance (ESR) and peptide synthesis fields, a fully active spin-labeled peptide hormone was reported. The ESR spectra of this alpha-melanocyte stimulating hormone (alpha-MSH) analogue (acetyl-Toac0-alpha-MSH) where Toac is the paramagnetic amino acid probe 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid, suggested a pH-independent conformation and a more restricted movement comparatively to the free Toac. Owing to its equivalent biological potency in a skin pigmentation assay as compared to the native alpha-MSH and its unique characteristic (paramagnetic, naturally fluorescent and fully active), this analogue is of great potential for investigation of relevant physiological roles reported for alpha-MSH.

Assuntos

alfa-MSH/síntese química; Espectroscopia de Ressonância de Spin Eletrônica; Conformação Proteica; Marcadores de Spin; alfa-MSH/química; alfa-MSH/metabolismo

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Alfa-MSH Idioma: En Revista: FEBS Lett Ano de publicação: 1999 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido

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