A kinetic study of some factors which influence the intestinal absorption of calcium in rats
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;24(2): 215-7, 1991. tab
Article
em En
| LILACS
| ID: lil-99459
Biblioteca responsável:
BR26.1
ABSTRACT
Rats weighing 100 ñ 8 g were used in an in vivo kinetic study of the intestinal absorption of calcium (CaA) from there systems 1) CaCl2, 2) whole cow's milk (M), and 3) a taple Brazilian diet (SBD). Food substrates were treated with HCl, neutralized and diluted with 0.15 mM NaCl to different concentrations. Each dilution was assayed by introducing 2 ml into the small intestine and the final amount of Ca*+ in the small intestine was determined 30 min later. The values for velocity (v= Vmax (Ca*+ in the small intestine was determined 30 min later. The values for velocity (v+Vmax (Ca*+)/(Ca*+) + Km) and affinity (1/Km) for the food substrates were 1.35, 15.51 and 2.31 *g Ca*+/min and 0.29, 0,016 and 0.266 *g Ca*+/ml, respectively. We conclude that the higher affinities observed for CaCl2 and SBD were not correlated with velocity. Factors modifying CaA appear to be protein (stimulator) and phylate and fiber (inhibitors)
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Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Cálcio
/
Absorção Intestinal
/
Intestino Delgado
Limite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1991
Tipo de documento:
Article
/
Congress and conference
País de publicação:
Brasil