Thin-section and freeze-fracture study of post-mortem chenges in dog myocardium
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;22(11): 1371-9, 1989. ilus
Article
em En
| LILACS
| ID: lil-83141
Biblioteca responsável:
BR26.1
ABSTRACT
Fragments of dog hearts submitted to 1,6,10,24 and 48 h of autolysis at 20-C were studied with freeze-fracture and thin-section techniques under the transmission electron microscope. The freeze-fracture replicas revealed maximal reduction in the mean number and clustering of intramembrane particles at 6 h post mortem, indicating irreversible cellular damage. However, sings of lethal damage (intramitochondrial amorphous dense bodies) were not observed in thin sections of the same material. The present study indicates that signs of irreversible damage similar to that occuring in in vivo ischemic alterations can be detected earlier by the freeze-fracture technique than by the thin-section technique
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Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Técnica de Fratura por Congelamento
/
Microtomia
/
Infarto do Miocárdio
/
Miocárdio
Limite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1989
Tipo de documento:
Article
País de publicação:
Brasil