Your browser doesn't support javascript.
loading
Effects of fluoride on proliferation and mineralization in periodontal ligament cells in vitro
Li, K Q; Jia, S S; Ma, M; Shen, H Z; Xu, L; Liu, G P; Huang, S Y; Zhang, D S.
Afiliação
  • Li, K Q; Shandong University. Department of Oral and Maxillofacial Surgery. Jinan. CN
  • Jia, S S; Shandong University. Department of Oral and Maxillofacial Surgery. Jinan. CN
  • Ma, M; Shandong University. Department of Oral and Maxillofacial Surgery. Jinan. CN
  • Shen, H Z; Shandong University. Department of Oral and Maxillofacial Surgery. Jinan. CN
  • Xu, L; Shandong University. Department of Oral and Maxillofacial Surgery. Jinan. CN
  • Liu, G P; Shandong University. Department of Oral and Maxillofacial Surgery. Jinan. CN
  • Huang, S Y; Shandong University. Department of Oral and Maxillofacial Surgery. Jinan. CN
  • Zhang, D S; Shandong University. Department of Oral and Maxillofacial Surgery. Jinan. CN
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;49(8): e5291, 2016. tab, graf
Article em En | LILACS | ID: lil-787385
Biblioteca responsável: BR1.1
ABSTRACT
Fluoride, which is often added to toothpaste or mouthwash in order to protect teeth from decay, may be a novel therapeutic approach for acceleration of periodontal regeneration. Therefore, we investigated the effects of fluoride on proliferation and mineralization in human periodontal ligament cells in vitro. The periodontal ligament cells were stimulated with various concentrations of NaF added into osteogenic inductive medium. Immunohistochemistry of cell identification, cell proliferation, alkaline phosphatase (ALP) activity assay, Alizarin red S staining and quantitative real-time-polymerase chain reaction (RT-PCR) were performed. Moderate concentrations of NaF (50-500 μmol/L) had pro-proliferation effects, while 500 μmol/L had the best effects. ALP activity and calcium content were significantly enhanced by 10 μmol/L NaF with osteogenic inductive medium. Quantitative RT-PCR data varied in genes as a result of different NaF concentrations and treatment periods. We conclude that moderate concentrations of NaF can stimulate proliferation and mineralization in periodontal ligament cells. These in vitro findings may provide a novel therapeutic approach for acceleration of periodontal regeneration by addition of suitable concentrations of NaF into the medication for periodontitis treatment, i.e., into periodontal packs and tissue patches.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Ligamento Periodontal / Fluoreto de Sódio / Proliferação de Células Tipo de estudo: Prognostic_studies Limite: Adolescent / Adult / Child / Humans Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Ligamento Periodontal / Fluoreto de Sódio / Proliferação de Células Tipo de estudo: Prognostic_studies Limite: Adolescent / Adult / Child / Humans Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: China País de publicação: Brasil