Purification and characterization of aminopeptidase activity from rat urine
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;20(6): 759-61, 1987. tab
Article
em En
| LILACS
| ID: lil-77431
Biblioteca responsável:
BR26.1
ABSTRACT
The aminopeptidase activity of rats urine was tested using L-aminoacy1-2-naphtylamides and L-Leu-p-nitroanilide. The enzyme preparation was purified by ion-exchange and gel filtration chromatography and showed only a single active protein active protein band on 7.5% polyacrylamide gel electrophoresis. The enzyme was characterized by studying the effects of ions (divalent cations and chloride), chelating agents and -SH and -S-S- group reagents and by determining kinetic parameters (Km and Vmax for different substrates and K1 for amino acids, antibiotics and anti-inflammatory drugs)
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Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Aminopeptidases
Limite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1987
Tipo de documento:
Article
/
Congress and conference
País de publicação:
Brasil