Expression of BAFF and BR3 in patients with systemic lupus erythematosus
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;49(3): e4853, Mar. 2016. tab, graf
Article
em En
| LILACS
| ID: lil-771933
Biblioteca responsável:
BR1.1
ABSTRACT
The objective of this study was to examine the relationship between the expression of B cell activating factor (BAFF) and BAFF receptor in patients with disease activity of systemic lupus erythematosus (SLE). Real-time RT-PCR was used to examine BAFF mRNA expression in peripheral blood monocytes of active and stable SLE patients and healthy controls. The percentage of BAFF receptor 3 (BR3) on B lymphocytes was measured by flow cytometry. Soluble BAFF levels in serum were assayed by ELISA. Microalbumin levels were assayed by an automatic immune analysis machine. BAFF mRNA and soluble BAFF levels were highest in the active SLE group, followed by the stable SLE group, and controls (P<0.01). The percentage of BR3 on B lymphocytes was downregulated in the active SLE group compared with the stable SLE group and controls (P<0.01). BAFF mRNA levels and soluble BAFF levels were higher in patients who were positive for proteinuria than in those who were negative (P<0.01). The percentage of BR3 on B lymphocytes was lower in patients who were positive for proteinuria than in those who were negative (P<0.01). The BAFF/BR3 axis may be over-activated in SLE patients. BAFF and BR3 levels may be useful parameters for evaluating treatment.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Fator Ativador de Células B
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Receptor do Fator Ativador de Células B
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Lúpus Eritematoso Sistêmico
Limite:
Adolescent
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Adult
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Female
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Humans
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Male
Idioma:
En
Revista:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Assunto da revista:
BIOLOGIA
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MEDICINA
Ano de publicação:
2016
Tipo de documento:
Article
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Project document
País de afiliação:
China
País de publicação:
Brasil