Kinetic properties of Streptomyces canarius L- Glutaminase and its anticancer efficiency
Braz. j. microbiol
; Braz. j. microbiol;46(4): 957-968, Oct.-Dec. 2015. tab, graf
Article
em En
| LILACS
| ID: lil-769664
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract L-glutaminase was produced by Streptomyces canarius FR (KC460654) with an apparent molecular mass of 44 kDa. It has 17.9 purification fold with a final specific activity 132.2 U/mg proteins and 28% yield recovery. The purified L-glutaminase showed a maximal activity against L-glutamine when incubated at pH 8.0 at 40 °C for 30 min. It maintained its stability at wide range of pH from 5.0 11.0 and thermal stable up to 60 °C with Tm value 57.5 °C. It has high affinity and catalytic activity for L-glutamine (Km 0.129 mM, Vmax 2.02 U/mg/min), followed by L-asparagine and L-aspartic acid. In vivo, L-glutaminase showed no observed changes in liver; kidney functions; hematological parameters and slight effect on RBCs and level of platelets after 10 days of rabbit's injection. The anticancer activity of L-glutaminase was also tested against five types of human cancer cell lines using MTT assay in vitro. L-glutaminase has a significant efficiency against Hep-G2 cell (IC50, 6.8 μg/mL) and HeLa cells (IC50, 8.3 μg/mL), while the growth of MCF-7 cells was not affected. L-glutaminase has a moderate cytotoxic effect against HCT-116 cell (IC50, 64.7 μg/mL) and RAW 264.7 cell (IC50, 59.3 μg/mL).
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Streptomyces
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Especificidade por Substrato
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Estabilidade Enzimática
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Humanos
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Cinética
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Células HeLa
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Proliferação de Células
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Biocatálise
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Glutaminase
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Glutamina
Idioma:
En
Revista:
Braz. j. microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Egito
País de publicação:
Brasil