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The effects of 6-gingerol on proliferation, differentiation, and maturation of osteoblast-like MG-63 cells
Fan, J.Z.; Yang, X.; Bi, Z.G..
Afiliação
  • Fan, J.Z.; Harbin Medicine University. First Affiliated Hospital. Department of Orthopedic Surgery. Harbin. CN
  • Yang, X.; Harbin Medicine University. First Affiliated Hospital. Department of Orthopedic Surgery. Harbin. CN
  • Bi, Z.G.; Harbin Medicine University. First Affiliated Hospital. Department of Orthopedic Surgery. Harbin. CN
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;48(7): 637-643, 07/2015. tab, graf
Article em En | LILACS | ID: lil-751350
Biblioteca responsável: BR1.1
ABSTRACT
We investigated whether 6-gingerol affects the maturation and proliferation of osteoblast-like MG63 cells in vitro. Osteoblast-like MG63 cells were treated with 6-gingerol under control conditions, and experimental inflammation was induced by tumor necrosis factor-α (TNF-α). Expression of different osteogenic markers and cytokines was analyzed by real-time PCR, Western blotting, and enzyme-linked immunosorbent assay. In addition, alkaline phosphatase (ALP) enzyme activity and biomineralization as markers for differentiation were measured. Treatment with 6-gingerol resulted in insignificant effects on the proliferation rate. 6-Gingerol induced the differentiation of osteoblast-like cells with increased transcription levels of osteogenic markers, upregulated ALP enzyme activity, and enhanced mineralized nodule formation. Stimulation with TNF-α led to enhanced interleukin-6 and nuclear factor-κB expression and downregulated markers of osteoblastic differentiation. 6-Gingerol reduced the degree of inflammation in TNF-α-treated MG-63 cells. In conclusion, 6-gingerol stimulated osteoblast differentiation in normal physiological and inflammatory settings, and therefore, 6-gingerol represents a promising agent for treating osteoporosis or bone inflammation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Trissomia / Síndrome de Down / Recém-Nascido de muito Baixo Peso / Transtornos Cromossômicos Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Female / Humans / Male / Newborn Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
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Imprimir
XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Trissomia / Síndrome de Down / Recém-Nascido de muito Baixo Peso / Transtornos Cromossômicos Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Female / Humans / Male / Newborn Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: China País de publicação: Brasil