Molecular characterization and epidemiology of cefoxitin resistance among Enterobacteriaceae lacking inducible chromosomal ampC genes from hospitalized and non-hospitalized patients in Algeria: description of new sequence type in Klebsiella pneumoniae isolates
Braz. j. infect. dis
; Braz. j. infect. dis;19(2): 187-195, Mar-Apr/2015. tab
Article
em En
| LILACS
| ID: lil-746522
Biblioteca responsável:
BR1.1
ABSTRACT
In this study, 922 consecutive non-duplicate clinical isolates of Enterobacteriaceae obtained from hospitalized and non-hospitalized patients at Bejaia, Algeria were analyzed for AmpC-type β-lactamases production. The ampC genes and their genetic environment were characterized using polymerase chain reaction (PCR) and sequencing. Plasmid incompatibility groups were determined by using PCR-based replicon typing. Phylogenetic grouping and multilocus sequence typing were determined for molecular typing of the plasmid-mediated AmpC (pAmpC) isolates.Of the isolates, 15 (1.6%) were identified as AmpC producers including 14 CMY-4- producing isolates and one DHA-1-producing Klebsiella pneumoniae. All AmpC-producing isolates co-expressed the broad-spectrum TEM-1 β-lactamase and three of them co-produced CTX-M and/or SHV-12 ESBL. Phylogenetic grouping and virulence genotyping of the E. coli isolates revealed that most of them belonged to groups D and B1. Multilocus sequence typing analysis of K. pneumoniae isolates identified four different sequence types (STs) with two new sequences ST1617 and ST1618. Plasmid replicon typing indicates that bla CMY-4 gene was located on broad host range A/C plasmid, while LVPK replicon was associated with bla DHA-1. All isolates carrying bla CMY-4 displayed the transposon-like structures ISEcp1/AISEcp1-blaCMY-blc-sugE. Our study showed that CMY-4 was the main pAmpC in the Enterobacteriaceae isolates inAlgeria.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Cefoxitina
/
Enterobacteriaceae
/
Klebsiella pneumoniae
/
Antibacterianos
Tipo de estudo:
Screening_studies
Limite:
Humans
País/Região como assunto:
Africa
Idioma:
En
Revista:
Braz. j. infect. dis
Assunto da revista:
DOENCAS TRANSMISSIVEIS
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Argélia
País de publicação:
Brasil