Your browser doesn't support javascript.
loading
Optimization of Bacillus subtilis cell growth effecting jiean-peptide production in fed batch fermentation using central composite design
Zhong, Juan; Zhang, Xiaoyong; Ren, Yanli; Yang, Jie; Tan, Hong; Zhou, Jinyan.
Afiliação
  • Zhong, Juan; Chengdu Institute of Biology. Key Laboratory of Environmental and Applied Microbiology. Chengdu. CN
  • Zhang, Xiaoyong; Chengdu Institute of Biology. Key Laboratory of Environmental and Applied Microbiology. Chengdu. CN
  • Ren, Yanli; Chengdu Institute of Biology. Key Laboratory of Environmental and Applied Microbiology. Chengdu. CN
  • Yang, Jie; Chengdu Institute of Biology. Key Laboratory of Environmental and Applied Microbiology. Chengdu. CN
  • Tan, Hong; Chengdu Institute of Biology. Key Laboratory of Environmental and Applied Microbiology. Chengdu. CN
  • Zhou, Jinyan; Chengdu Institute of Biology. Key Laboratory of Environmental and Applied Microbiology. Chengdu. CN
Electron. j. biotechnol ; Electron. j. biotechnol;17(3): 132-136, May 2014. ilus, graf
Article em En | LILACS | ID: lil-719103
Biblioteca responsável: CL1.1
ABSTRACT
Background Optimization of nutrient feeding was developed to improve the growth of Bacillus subtilis in fed batch fermentation to increase the production of jiean-peptide (JAA). A central composite design (CCD) was used to obtain a model describing the relationship between glucose, total nitrogen, and the maximum cell dry weight in the culture broth with fed batch fermentation in a 5 L fermentor. Results The results were analyzed using response surface methodology (RSM), and the optimized values of glucose and total nitrogen concentration were 30.70 g/L and 1.68 g/L in the culture, respectively. The highest cell dry weight was improved to 77.50 g/L in fed batch fermentation, which is 280% higher than the batch fermentation concentration (20.37 g/L). This led to a 44% increase of JAA production in fed batch fermentation as compared to the production of batch fermentation. Conclusion The results of this work improve the present production of JAA and may be adopted for other objective products' production.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Peptídeos / Bacillus subtilis Idioma: En Revista: Electron. j. biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Chile
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Peptídeos / Bacillus subtilis Idioma: En Revista: Electron. j. biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Chile