Anti-Mycobacterium tuberculosis activity and cytotoxicity of Calophyllum brasiliense Cambess (Clusiaceae)

Pires, Claudia Terencio Agostinho; Brenzan, Mislaine Adriana; Scodro, Regiane Bertin de Lima; Cortez, Diógenes Aparício Garcia; Lopes, Luciana Dias Ghiraldi; Siqueira, Vera Lucia Dias; Cardoso, Rosilene Fressatti

Pires, Claudia Terencio Agostinho; Brenzan, Mislaine Adriana; Scodro, Regiane Bertin de Lima; Cortez, Diógenes Aparício Garcia; Lopes, Luciana Dias Ghiraldi; Siqueira, Vera Lucia Dias; Cardoso, Rosilene Fressatti.

Afiliação

Pires, Claudia Terencio Agostinho; aff1.
Brenzan, Mislaine Adriana; aff1.
Scodro, Regiane Bertin de Lima; aff1.
Cortez, Diógenes Aparício Garcia; aff1.
Lopes, Luciana Dias Ghiraldi; aff1.
Siqueira, Vera Lucia Dias; aff1.
Cardoso, Rosilene Fressatti; aff1.

Mem. Inst. Oswaldo Cruz ; 109(3): 324-329, 06/2014. tab, graf

Article em En | LILACS | ID: lil-711741

Biblioteca responsável: BR1.1

ABSTRACT

ABSTRACT

We evaluated the in vitro anti-Mycobacterium tuberculosis activity and the cytotoxicity of dichloromethane extract and pure compounds from the leaves of Calophyllum brasiliense. Purification of the dichloromethane extract yielded the pure compounds (-) mammea A/BB (1), (-) mammea B/BB (2) and amentoflavone (3). The compound structures were elucidated on the basis of spectroscopic and spectrometric data. The contents of bioactive compounds in the extracts were quantified using high performance liquid chromatography coupled to an ultraviolet detector. The anti-M. tuberculosis activity of the extracts and the pure compounds was evaluated using a resazurin microtitre assay plate. The cytotoxicity assay was performed in J774G.8 macrophages using the 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colourimetric method. The quantification of the dichloromethane extract showed (1) and (2) at concentrations of 31.86 ± 2.6 and 8.24 ± 1.1 µg/mg of extract, respectively. The dichloromethane and aqueous extracts showed anti-M. tuberculosis H37Rv activity of 62.5 and 125 µg/mL, respectively. Coumarins (1) and (2) showed minimal inhibitory concentration ranges of 31.2 and 62.5 µg/mL against M. tuberculosis H37Rv and clinical isolates. Compound (3) showed no activity against M. tuberculosis H37Rv. The selectivity index ranged from 0.59-1.06. We report the activity of the extracts and coumarins from the leaves of C. brasiliense against M. tuberculosis.

Assuntos

Antibacterianos/farmacologia; Biflavonoides/farmacologia; Calophyllum/química; Macrófagos/efeitos dos fármacos; Cloreto de Metileno/farmacologia; Mycobacterium tuberculosis/efeitos dos fármacos; Extratos Vegetais/farmacologia; Antibacterianos/toxicidade; Biflavonoides/isolamento & purificação; Biflavonoides/toxicidade; Testes de Sensibilidade Microbiana; Cloreto de Metileno/isolamento & purificação; Cloreto de Metileno/toxicidade; Extratos Vegetais/toxicidade

Palavras-chave

Calophyllum brasiliense; Coumarin; Cytotoxicity - 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide; Mycobacterium tuberculosis

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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Extratos Vegetais / Calophyllum / Biflavonoides / Macrófagos / Cloreto de Metileno / Antibacterianos / Mycobacterium tuberculosis País/Região como assunto: America do sul / Brasil Idioma: En Revista: Mem. Inst. Oswaldo Cruz Assunto da revista: MEDICINA TROPICAL / PARASITOLOGIA Ano de publicação: 2014 Tipo de documento: Article País de publicação: Brasil

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