The potential effects of Zataria multiflora Boiss essential oil on growth, aflatoxin production and transcription of aflatoxin biosynthesis pathway genes of toxigenic Aspergillus parasiticus

Yahyaraeyat, R.; Khosravi, A.R.; Shahbazzadeh, D.; Khalaj, V.

Yahyaraeyat, R.; Khosravi, A.R.; Shahbazzadeh, D.; Khalaj, V..

Afiliação

Yahyaraeyat, R.; University of Tehran. Faculty of Veterinary Medicine. Mycology Research Centre. Tehran. IR
Khosravi, A.R.; University of Tehran. Faculty of Veterinary Medicine. Mycology Research Centre. Tehran. IR
Shahbazzadeh, D.; University of Tehran. Faculty of Veterinary Medicine. Mycology Research Centre. Tehran. IR
Khalaj, V.; University of Tehran. Faculty of Veterinary Medicine. Mycology Research Centre. Tehran. IR

Braz. j. microbiol ; Braz. j. microbiol;44(2): 649-655, 2013. ilus, tab

Article em En | LILACS | ID: lil-688596

Biblioteca responsável: BR1.1

ABSTRACT

ABSTRACT

This study aims at evaluating the effects of Zataria multiflora (Z. multiflora) essential oil (EO) on growth, aflatoxin production and transcription of aflatoxin biosynthesis pathway genes. Total RNAs of Aspergillus parasiticus (A.parasiticus) ATCC56775 grown in yeast extract sucrose (YES) broth medium treated with Z. multiflora EO were subjected to reverse transcription-polymerase chain reaction (RT-PCR). Specific primers of nor-1, ver-1, omt-A and aflR genes were used. In parallel mycelial dry weight of samples were measured and all the media were assayed by high-pressure liquid chromatography (HPLC) for aflatoxinB1 (AFB1), aflatoxinB2 (AFB2), aflatoxinG1 (AFG1), aflatoxinG2 (AFG2) and aflatoxin total (AFTotal) production. The results showed that mycelial dry weight and aflatoxin production reduce in the presence of Z. multiflora EO (100 ppm) on day 5 of growth. It was found that the expression of nor-1, ver-1, omt-A and aflR genes was correlated with the ability of fungus to produce aflatoxins on day 5 in YES medium. RT-PCR showed that in the presence of Z.multiflora EO (100 ppm) nor-1, ver-1 and omtA genes expression was reduced. It seems that toxin production inhibitory effects of Z. multiflora EO on day 5 may be at the transcription level and this herb may cause reduction in aflatoxin biosynthesis pathway genes activity.

Assuntos

Aflatoxinas/biossíntese; Antifúngicos/metabolismo; Aspergillus/efeitos dos fármacos; Vias Biossintéticas/efeitos dos fármacos; Lamiaceae/química; Óleos Voláteis/metabolismo; Transcrição Gênica/efeitos dos fármacos; Antifúngicos/isolamento & purificação; Aspergillus/genética; Aspergillus/crescimento & desenvolvimento; Aspergillus/metabolismo; Vias Biossintéticas/genética; Cromatografia Líquida de Alta Pressão; Perfilação da Expressão Gênica; Óleos Voláteis/isolamento & purificação; Reação em Cadeia da Polimerase Via Transcriptase Reversa

Palavras-chave

Aflatoxin; Aspergillus parasiticus; Reverse transcription-polymerase chain reaction; Zataria multiflora

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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Aspergillus / Transcrição Gênica / Óleos Voláteis / Lamiaceae / Aflatoxinas / Vias Biossintéticas / Antifúngicos Idioma: En Revista: Braz. j. microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Irã País de publicação: Brasil

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