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Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition
Khan, Abdul Basit; Khattak, Mehmood Iqbal; Tarar, Omer Mukhtar; Habib, Farzana; Jamil, Khalid; Yasmin, Arfa; Parvez, Shoukat.
Afiliação
  • Khan, Abdul Basit; Food and Marine Resources Research Centre. PCSIR Laboratories Complex. Karachi. PK
  • Khattak, Mehmood Iqbal; Food and Marine Resources Research Centre. PCSIR Laboratories Complex. Karachi. PK
  • Tarar, Omer Mukhtar; Food and Marine Resources Research Centre. PCSIR Laboratories Complex. Karachi. PK
  • Habib, Farzana; Food and Marine Resources Research Centre. PCSIR Laboratories Complex. Karachi. PK
  • Jamil, Khalid; Food and Marine Resources Research Centre. PCSIR Laboratories Complex. Karachi. PK
  • Yasmin, Arfa; Food and Marine Resources Research Centre. PCSIR Laboratories Complex. Karachi. PK
  • Parvez, Shoukat; Food and Marine Resources Research Centre. PCSIR Laboratories Complex. Karachi. PK
Braz. arch. biol. technol ; Braz. arch. biol. technol;56(4): 645-652, July-Aug. 2013. ilus, graf, tab
Article em En | LILACS | ID: lil-684517
Biblioteca responsável: BR1.1
ABSTRACT
In this study, a variety of samples were screened for the presence of PHA synthase gene. Results showed that 16 out of 102 isolated were positive for PHA respective genes. The highest prevalence was observed in Pseudomonas aeruginosa. The capability of PHA production was also shown by growing these strains on the defined medium and subsequent analysis using intracellular granules staining and Fourier transform infrared spectroscopy (FT-IR). The microscopic analysis showed that the positive strains accumulated PHA in the cell. The FT-IR analysis showed the presence of PHA peaks in the dried cells as well as in extraction product. P aeruginosa strain P7 showed higher concentration of PHA compared to the others as demonstrated by the highest respective peaks in FT-IR.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Tipo de estudo: Risk_factors_studies Idioma: En Revista: Braz. arch. biol. technol Assunto da revista: BIOLOGIA Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Paquistão País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Tipo de estudo: Risk_factors_studies Idioma: En Revista: Braz. arch. biol. technol Assunto da revista: BIOLOGIA Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Paquistão País de publicação: Brasil