Molecular characterization of Streptococcus pneumoniae serotype 1 invasive isolates in Colombia / Caracterización molecular de cepas invasoras de Streptococcus pneumoniae serotipo 1 aisladas en Colombia
Rev. panam. salud pública
; 33(6): 422-426, Jun. 2013. graf, tab
Article
em En
| LILACS
| ID: lil-682470
Biblioteca responsável:
BR1.1
ABSTRACT
OBJECTIVE:
To determine the genetic relationship between Streptococcus pneumoniae serotype 1 Colombian isolates recovered from invasive disease between 1994 and 2011 and recognized serotype 1 international clones.METHODS:
A total of 135 S. pneumoniae serotype 1 isolates with epidemiological and antimicrobial susceptibility data (Clinical and Laboratory Standards Institute, 2012) were studied. The genetic relationship with recognized international clones was established by pulsed-field gel electrophoresis (PFGE) with SmaI restriction enzyme. Multilocus sequence typing (MLST) was standardized to determine the sequence type (ST) in seven isolates representing different clonal groups. Control and reference strain R6, and clones Sweden¹ ST217, Sweden¹ ST304, Sweden¹ ST306, and USA¹ ST615, were used.RESULTS:
PFGE revealed that 89.7% of the isolates were associated with Sweden¹ ST306, 3.7% were associated with Sweden¹ ST304, and 6.6% were not clonally related. Using MLST, ST306 was confirmed in six isolates and ST304 in one.CONCLUSIONS:
In contrast to Brazil and the United States, where clones Sweden¹ ST304 and ST227 prevail, invasive disease caused by S. pneumoniae serotype 1 in Colombia is principally associated with the dispersion of isolates related to clone Sweden¹ ST306.RESUMEN
OBJETIVO:
Determinar la relación genética entre las cepas de Streptococcus pneumoniae serotipo 1 aisladas en Colombia en casos de enfermedad invasora entre 1994 y 2011 y los clones internacionales reconocidos del serotipo 1.MÉTODOS:
Se estudiaron un total de 135 cepas de S. pneumoniae serotipo 1 de las que se tenían datos epidemiológicos y de sensibilidad a los antimicrobianos (Clinical and Laboratory Standards Institute, 2012). Se estableció su relación genética con los clones internacionales reconocidos mediante electroforesis en gel de campo pulsátil (PFGE) utilizando la enzima de restricción SmaI. Se estandarizó la tipificación de secuencias mulitlocus (MLST) para determinar el tipo de secuencia (ST) en siete cepas que representaban diferentes grupos clonales. Se utilizaron la cepa de control y referencia R6 y los clones Sweden¹ ST217, Sweden¹ ST304, Sweden¹ ST306, y USA¹ ST615.RESULTADOS:
La PFGE reveló que 89,7% de las cepas se asociaban con Sweden¹ ST306, 3,7% con Sweden¹ ST304, y 6,6% no mostraron relación clonal. Mediante MLST, se confirmó la relación con ST306 en seis cepas y con ST304 en una.CONCLUSIONES:
A diferencia de Brasil y Estados Unidos, donde prevalecen los clones Sweden¹ ST304 y ST227, la enfermedad invasora causada por S. pneumoniae serotipo 1 en Colombia se asocia principalmente con la dispersión de cepas relacionadas con el clon Sweden¹ ST306.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Streptococcus pneumoniae
Tipo de estudo:
Guideline
Limite:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Child
/
Child, preschool
/
Humans
/
Infant
País/Região como assunto:
America do sul
/
Colombia
Idioma:
En
Revista:
Rev. panam. salud pública
Assunto da revista:
SAUDE PUBLICA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Colômbia
País de publicação:
Estados Unidos