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Proteomic data show an increase in autoantibodies and alpha-fetoprotein and a decrease in apolipoprotein A-II with time in sera from senescence-accelerated mice
Brazilian Journal of Medical and Biological Research; Guo, S.J.; Qi, C.H.; Zhou, W.X.; Zhang, Y.X.; Zhang, X.M.; Wang, J.; Wang, H.X..
Afiliação
  • Guo, S.J.; Beijing Institute of Pharmacology and Toxicology. Beijing. CN
  • Qi, C.H.; Beijing Institute of Pharmacology and Toxicology. Beijing. CN
  • Zhou, W.X.; Beijing Institute of Pharmacology and Toxicology. Beijing. CN
  • Zhang, Y.X.; Beijing Institute of Pharmacology and Toxicology. Beijing. CN
  • Zhang, X.M.; Beijing Institute of Pharmacology and Toxicology. Beijing. CN
  • Wang, J.; Beijing Institute of Pharmacology and Toxicology. Beijing. CN
  • Wang, H.X.; Beijing Institute of Pharmacology and Toxicology. Beijing. CN
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;46(5): 417-425, maio 2013. tab, graf
Article em En | LILACS | ID: lil-675669
Biblioteca responsável: BR1.1
ABSTRACT
We evaluated changes in levels by comparing serum proteins in senescence-accelerated mouse-prone 8 (SAMP8) mice at 2, 6, 12, and 15 months of age (SAMP8-2 m, -6 m, -12 m, -15 m) to age-matched SAM-resistant 1 (SAMR1) mice. Mice were sacrificed, and blood was analyzed by 2-dimensional electrophoresis combined with mass spectrometry. Five protein spots were present in all SAMP8 serum samples, but only appeared in SAMR1 samples at 15 months of age except for spot 3, which also showed a slight expression in SAMR1-12 m sera. Two proteins decreased in the sera from SAMP8-2 m, -6 m, and -12 m mice, and divided into 2 spots each in SAMP8-15 m sera. Thus, the total number of altered spots in SAMP8 sera was 7; of these, 4 were identified as Ig kappa chain V region (M-T413), chain A of an activity suppressing Fab fragment to cytochrome P450 aromatase (32C2_A), alpha-fetoprotein, and apolipoprotein A-II. M-T413 is a monoclonal CD4 antibody, which inhibits T cell proliferation. We found that M-T413 RNA level was significantly enhanced in splenocytes from SAMP8-2 m mice. This agreed with serum M-T413 protein alterations and a strikingly lower blood CD4+ T cell count in SAMP8 mice when compared to the age-matched SAMR1 mice, with the latter negatively correlating with serum M-T413 protein volume. Age-related changes in serum proteins favored an increase in autoantibodies and alpha-fetoprotein and a decrease of apolipoprotein A-II, which occurred in SAMP8 mice at 2 months of age and onwards. These proteins may serve as candidate biomarkers for early aging.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Autoanticorpos / Envelhecimento / Alfa-Fetoproteínas / Apolipoproteína A-II / Estresse Oxidativo Limite: Animals Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2013 Tipo de documento: Article / Project document País de afiliação: China País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Autoanticorpos / Envelhecimento / Alfa-Fetoproteínas / Apolipoproteína A-II / Estresse Oxidativo Limite: Animals Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2013 Tipo de documento: Article / Project document País de afiliação: China País de publicação: Brasil