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A DNA pooling based system to detect Escherichia coli virulence factors in fecal and wastewater samples
Chacón J, Luz María; Taylor C, Lizeth; Valiente A, Carmen; Alvarado P, Irene; Cortés B, Ximena.
Afiliação
  • Chacón J, Luz María; University of Costa Rica. Instituto de Investigaciones en Salud. Sección Infección-Nutrición. Montes de Oca. CR
  • Taylor C, Lizeth; University of Costa Rica. Microbiology Department. Virology Division. Montes de Oca. CR
  • Valiente A, Carmen; Instituto Costarricense de Acueductos y Alcantarillados. Laboratorio Nacional de Aguas. La Unión. CR
  • Alvarado P, Irene; University of Costa Rica. Microbiology Department. Virology Division. Montes de Oca. CR
  • Cortés B, Ximena; University of Costa Rica. Microbiology Department. Virology Division. Montes de Oca. CR
Braz. j. microbiol ; Braz. j. microbiol;43(4): 1319-1326, Oct.-Dec. 2012. ilus, tab
Article em En | LILACS | ID: lil-665815
Biblioteca responsável: BR32.1
ABSTRACT
The availability of a useful tool for simple and timely detection of the most important virulent varieties of Escherichia coli is indispensable. To this end, bacterial DNA pools which had previously been categorized were obtained from isolated colonies as well as selected in terms of utilized phenotype; the pools were assessed by two PCR Multiplex for the detection of virulent E. coli eaeA, bfpA, stx1, stx2, ipaH, ST, LT, and aatA genes, with the 16S gene used as DNA control. The system was validated with 66 fecal samples and 44 wastewater samples. At least one positive isolate was detected by a virulent gene among the 20 that were screened. The analysis of fecal samples from children younger than 6 years of age detected frequencies of 25% LT positive strains, 8.3% eae, 8.3% bfpA, 16.7% ipaH, as well as 12.5 % aatA and ST. On the other hand, wastewater samples revealed frequencies of 25.7% eaeA positive, 30.3% stx1, 15.1% LT and 19.7% aatA. This study is an initial step toward carrying out epidemiological field research that will reveal the presence of these bacterial varieties.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Fenótipo / Surtos de Doenças / Técnicas Bacteriológicas / Escherichia coli / Infecções por Escherichia coli / Águas Residuárias / Genes Bacterianos Limite: Child Idioma: En Revista: Braz. j. microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Costa Rica País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Fenótipo / Surtos de Doenças / Técnicas Bacteriológicas / Escherichia coli / Infecções por Escherichia coli / Águas Residuárias / Genes Bacterianos Limite: Child Idioma: En Revista: Braz. j. microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Costa Rica País de publicação: Brasil