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Comparative evaluation of the microplate nitrate reductase assay and the rezasurin microtitre assay for the rapid detection of multidrug resistant Mycobacterium tuberculosis clinical isolates
Coban, Ahmet Yilmaz; Uzun, Meltem; Akgunes, Alper; Durupinar, Belma.
Afiliação
  • Coban, Ahmet Yilmaz; Ondokuz Mayis University. Medical School. Department of Medical Microbiology. Samsun. TR
  • Uzun, Meltem; Istanbul University. Istanbul Medical School. Department of Medical Microbiology. Istanbul. TR
  • Akgunes, Alper; Samsun Chest Diseases Hospital. Medical Microbiology Laboratory. Samsun. TR
  • Durupinar, Belma; Ondokuz Mayis University. Medical School. Department of Medical Microbiology. Samsun. TR
Mem. Inst. Oswaldo Cruz ; 107(5): 578-581, Aug. 2012. ilus, tab
Article em En | LILACS | ID: lil-643741
Biblioteca responsável: BR1.1
ABSTRACT
The microplate nitrate reductase assay (MNRA) and the rezasurin microtitre assay (REMA) were used for the susceptibility testing of 73 clinical isolates and the results were compared with those that were obtained using the Bactec 460 TB and Bactec MGIT 960 systems. The REMA and the MNRA were performed in 96-well plates. For the REMA, the concentrations of isoniazid (INH) and rifampicin (RIF) ranged from 1.0-0.01 µg/mL and 2.0-0.03 µg/mL, respectively. For the MNRA, the INH concentration was between 1.0-0.03 µg/mL and the RIF concentration was between 2.0-0.06 µg/mL. For the MNRA, the sensitivity, specificity, positive predictive value, negative predictive value and INH/RIF agreement were 100/95.6, 97.6/100, 96.8/100, 100/98 and 98.6/98.6, respectively, and for the REMA, they were 100/91.3, 90.4/100, 88.5/100, 100/96.1 and 94.5/97.2, respectively. Our data suggest that these two rapid, low-cost methods may be inexpensive, alternative assays for the rapid detection of multidrug resistant tuberculosis in low-income countries.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Rifampina / Testes de Sensibilidade Microbiana / Farmacorresistência Bacteriana Múltipla / Isoniazida / Mycobacterium tuberculosis / Antituberculosos Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Mem. Inst. Oswaldo Cruz Assunto da revista: MEDICINA TROPICAL / PARASITOLOGIA Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Turquia País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Rifampina / Testes de Sensibilidade Microbiana / Farmacorresistência Bacteriana Múltipla / Isoniazida / Mycobacterium tuberculosis / Antituberculosos Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Mem. Inst. Oswaldo Cruz Assunto da revista: MEDICINA TROPICAL / PARASITOLOGIA Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Turquia País de publicação: Brasil