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Epstein-Barr virus DNA load and its association with Helicobacter pylori infection in gastroduodenal diseases
Shukla, Sanket Kumar; Prasad, K. N; Tripathi, Aparna; Singh, Avinash; Saxena, Ashish; Ghoshal, Uday Chand; Krishnani, Narendra; Husain, Nuzhat.
Afiliação
  • Shukla, Sanket Kumar; Sanjay Gandhi Postgraduate Institute of Medical Sciences. Department of Microbiology. IN
  • Prasad, K. N; Sanjay Gandhi Postgraduate Institute of Medical Sciences. Department of Microbiology. IN
  • Tripathi, Aparna; Sanjay Gandhi Postgraduate Institute of Medical Sciences. Department of Microbiology. IN
  • Singh, Avinash; Sanjay Gandhi Postgraduate Institute of Medical Sciences. Department of Microbiology. IN
  • Saxena, Ashish; University of Texas Medical Branch. Department of Biochemistry and Molecular Biology. US
  • Ghoshal, Uday Chand; s.af
  • Krishnani, Narendra; Sanjay Gandhi Postgraduate Institute of Medical Sciences. Department of Pathology. IN
  • Husain, Nuzhat; Sanjay Gandhi Postgraduate Institute of Medical Sciences. Department of Microbiology. IN
Braz. j. infect. dis ; Braz. j. infect. dis;15(6): 583-590, Nov.-Dec. 2011. ilus, tab
Article em En | LILACS | ID: lil-610531
Biblioteca responsável: BR1.1
ABSTRACT
Helicobacter pylori and Epstein-Barr virus (EBV) infections are common worldwide. Although H. pylori infection is a major factor in gastroduodenal diseases, its role in association with EBV infection is unknown.

Objective:

To study the association of H. pylori infection and EBV DNA load in patients with gastroduodenal diseases.

Methods:

Biopsy samples were collected from 200 adult patients [non-ulcer dyspepsia (NUD) 100, peptic ulcer disease (PUD) 50, gastric carcinoma (GC) 50] undergoing upper gastrointestinal endoscopy. H. pylori infection was diagnosed by rapid urease test, culture, histopathology, PCR and Q-PCR. EBV DNA was detected by non-polymorphic Epstein-Barr nuclear antigen-1 (EBNA-1) gene based Q-PCR.

Results:

In patients with GC and PUD, EBV DNA was detected more often than NUD (GC versus NUD = 90 percent versus 37 percent, p < 0.001; PUD versus NUD = 70 percent versus 37 percent, p < 0.001). The dual prevalence of H. pylori infection and EBV DNA was significantly higher in patients with GC and PUD than in those with NUD. Median copy number of EBV DNA was considerably higher in GC and PUD than NUD (p < 0.01). The copy number of EBV DNA was significantly higher in H. pylori infected patients (p = 0.015). The number of ureA gene copies was also found to be significantly higher in PUD and NUD with presence of EBV DNA. However, in GC no significant difference was seen between EBV positive and negative status.

Conclusion:

There was a trend for higher EBV DNA load in H. pylori positive individuals suggesting a probable role of H. pylori in modulating the conversion of EBV to its lytic phase.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Úlcera Péptica / Neoplasias Gástricas / DNA Viral / Helicobacter pylori / Infecções por Helicobacter / Infecções por Vírus Epstein-Barr Tipo de estudo: Diagnostic_studies / Risk_factors_studies Limite: Adult / Female / Humans / Male Idioma: En Revista: Braz. j. infect. dis Assunto da revista: DOENCAS TRANSMISSIVEIS Ano de publicação: 2011 Tipo de documento: Article / Project document País de afiliação: Índia / Estados Unidos País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Úlcera Péptica / Neoplasias Gástricas / DNA Viral / Helicobacter pylori / Infecções por Helicobacter / Infecções por Vírus Epstein-Barr Tipo de estudo: Diagnostic_studies / Risk_factors_studies Limite: Adult / Female / Humans / Male Idioma: En Revista: Braz. j. infect. dis Assunto da revista: DOENCAS TRANSMISSIVEIS Ano de publicação: 2011 Tipo de documento: Article / Project document País de afiliação: Índia / Estados Unidos País de publicação: Brasil