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Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica
Cotârleţ, Mihaela; Negoiţã, Teodor Gh; Bahrim, Gabriela E; Stougaard, Peter.
Afiliação
  • Cotârleţ, Mihaela; Romanian Polar Research Institute. RO
  • Negoiţã, Teodor Gh; Romanian Polar Research Institute. RO
  • Bahrim, Gabriela E; University Dunãrea de Jos. Faculty of Food Science and Engineering. Bioengineering Department. RO
  • Stougaard, Peter; University of Copenhagen. Faculty of Life Sciences. Department of Agriculture and Ecology. DK
Braz. j. microbiol ; Braz. j. microbiol;42(3): 868-877, July-Sept. 2011. ilus, tab
Article em En | LILACS | ID: lil-607515
Biblioteca responsável: BR32.1
ABSTRACT
The aim of this study was to isolate novel enzyme-producing bacteria from vegetation samples from East Antarctica and also to characterize them genetically and biochemically in order to establish their phylogeny. The ability to grow at low temperature and to produce amylases and proteases cold-active was also tested. The results of the 16S rRNA gene sequence analysis showed that the 4 Alga rRNA was 100 percent identical to the sequences of Streptomyces sp. rRNA from Norway and from the Solomon Islands. The Streptomyces grew well in submerged system at 20ºC, cells multiplication up to stationary phase being drastically increased after 120 h of submerged cultivation. The beta-amylase production reached a maximum peak after seven days, while alpha-amylase and proteases were performing biosynthesis after nine days of submerged cultivation at 20ºC. Newly Streptomyces were able to produce amylase and proteases in a cold environment. The ability to adapt to low temperature of these enzymes could make them valuable ingredients for detergents, the food industry and bioremediation processes which require low temperatures.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Streptomyces / Beta-Amilase / RNA Bacteriano / Microbiologia Ambiental / Amilases Idioma: En Revista: Braz. j. microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2011 Tipo de documento: Article / Project document País de afiliação: Dinamarca / Romênia País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Streptomyces / Beta-Amilase / RNA Bacteriano / Microbiologia Ambiental / Amilases Idioma: En Revista: Braz. j. microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2011 Tipo de documento: Article / Project document País de afiliação: Dinamarca / Romênia País de publicação: Brasil