Your browser doesn't support javascript.
loading
Acutolysin C, a weak hemorrhagic toxin from the venom of Agkistrodon acutus with leucoagglutination activity
Wei, C. B; Chen, J; Li, J. H.
Afiliação
  • Wei, C. B; Western Anhui University, Lu'an. Department of Chemistry and Biology. Lu'an. CN
  • Chen, J; Ningbo University. Faculty of Life Science and Biotechnology. Ningbo. CN
  • Li, J. H; Anhui University. Life Science College. Hefei. CN
J. venom. anim. toxins incl. trop. dis ; J. venom. anim. toxins incl. trop. dis;17(1): 34-41, 2011. ilus, tab
Article em En | LILACS, VETINDEX | ID: lil-576880
Biblioteca responsável: BR68.1
ABSTRACT
The properties and agglutination activity of acutolysin C, a hemorrhagic metalloproteinase obtained from Agkistrodon acutus venom, were studied herein. Acutolysin C is a basic glycoprotein consisting of a single polypeptide chain with a molecular weight of 23.1 kDa and pI 8.7, containing one Zn2+ and one Ca2+ per molecule. It possesses caseinolytic, weak lethal (LD50 = 7.6 mg/kg) and weak hemorrhagic (MHD = 12.0 µg) activities, but does not present fibrinolytic, fibrinogenolytic, arginine esterase and phospholipase A2 actions. In addition, it revealed agglutination activity on some animal lymphocytes, including five species of mammals, six of birds, three of reptiles and one of amphibians, but had no effect on lymphocytes from two species of reptiles, one amphibian and nine species of fish. It had no effects on the erythrocytes and platelets of all 26 animal species tested. Both leucoagglutination and caseinolytic activities were inhibited by EDTA; while cysteine, 2-mercaptoethanol, 1,4-dithiothreitol, glutathione, serum against acutolysin C and serum against homologous snake venom as well as glucose, sucrose, mannose, lactose and galactose had no effects on inhibition. The lowest concentration of acutolysin C that induced mouse lymphocyte agglutination was 2.5 µg/mL. Acutolysin C is an interesting substance since it is the first member of the hemorrhagin family to be shown to have leucoagglutination activity. (AU)
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS / VETINDEX Assunto principal: Venenos de Serpentes / Agkistrodon / Aglutinação / Metaloproteases / Hemorragia Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: TOXICOLOGIA Ano de publicação: 2011 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS / VETINDEX Assunto principal: Venenos de Serpentes / Agkistrodon / Aglutinação / Metaloproteases / Hemorragia Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: TOXICOLOGIA Ano de publicação: 2011 Tipo de documento: Article País de afiliação: China País de publicação: Brasil