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The NRAMPI, VDR and TNF-¦Á gene polymorphisms in Iranian tuberculosis patients: the study on host susceptibility
Merza, Muayad; Farnia, Parissa; Anoosheh, Sabar; Varahram, Mohammed; Kazampour, Mehdi; Pajand, Omid; Saeif, Shima; Mirsaeidi, Mehdi; Masjedi, Mohammad Reza; Velayati, Ali Akbar; Hoffner, Sven.
Afiliação
  • Merza, Muayad; Shahid Beheshti University. National Research Institute of Tuberculosis and Lung Disease. Mycobacteriology Research Centre. Darabad. IR
  • Farnia, Parissa; Shahid Beheshti University. National Research Institute of Tuberculosis and Lung Disease. Mycobacteriology Research Centre. Darabad. IR
  • Anoosheh, Sabar; Shahid Beheshti University. National Research Institute of Tuberculosis and Lung Disease. Mycobacteriology Research Centre. Darabad. IR
  • Varahram, Mohammed; Shahid Beheshti University. National Research Institute of Tuberculosis and Lung Disease. Mycobacteriology Research Centre. Darabad. IR
  • Kazampour, Mehdi; Shahid Beheshti University. National Research Institute of Tuberculosis and Lung Disease. Mycobacteriology Research Centre. Darabad. IR
  • Pajand, Omid; Shahid Beheshti University. National Research Institute of Tuberculosis and Lung Disease. Mycobacteriology Research Centre. Darabad. IR
  • Saeif, Shima; Shahid Beheshti University. National Research Institute of Tuberculosis and Lung Disease. Mycobacteriology Research Centre. Darabad. IR
  • Mirsaeidi, Mehdi; Shahid Beheshti University. National Research Institute of Tuberculosis and Lung Disease. Mycobacteriology Research Centre. Darabad. IR
  • Masjedi, Mohammad Reza; Shahid Beheshti University. National Research Institute of Tuberculosis and Lung Disease. Mycobacteriology Research Centre. Darabad. IR
  • Velayati, Ali Akbar; Shahid Beheshti University. National Research Institute of Tuberculosis and Lung Disease. Mycobacteriology Research Centre. Darabad. IR
  • Hoffner, Sven; Swedish Institute for Infectious Disease control. Department of Bacteriology. Solna. SE
Braz. j. infect. dis ; Braz. j. infect. dis;13(4): 252-256, Aug. 2009. tab
Article em En | LILACS | ID: lil-539758
Biblioteca responsável: BR1.1
ABSTRACT
The natural resistance-associated macrophage protein (NRAMP1), Vitamin-D receptor (VDR) and Tumor necrosis factor (TNF-¦Á) have been associated in susceptibility to tuberculosis, but the results have been inconsistent. This study aimed to determine the association of NRAMP1, VDR, and TNF-¨¢ variant with development of pulmonary tuberculosis (PTB) among Iranian patients. The single nucleotide polymorphisms (SNPs) at INT4, D543, 3'UTR of NRAMP1 gene, SNPs in restriction sites of BsmI, and FokI of the VDR gene and SNPs of TNF-¦Á at -238,-308, -244,857,-863 positions were analyzed by PCR-RFLP among two groups of individual; patients with PTB (n=117) and healthy controls (n=60). Thereafter, the frequencies of extended haplotypes and diplotypes were estimated. No statistically significant differences were observed in allele frequencies of INT4, D543, 3'UTR of NRAMPI, FokI of VDR and TNF-¦Á at -238, -244,-863 and -857 position. Although, the frequency of b allele of BsmI [ORs 0.24 CI95 percent (0.07-0.67 (p=0.001)] and -308 A variant in TNF-¦Á promoter region [ORs0.26 CI95 percent( 0.07-0.77) (p=0.006)] were significantly more in PTB patients than healthy controls. The frequency of extended diplotypes of NRAMP [GG TGTG++GA; 0.02(0.001-0.0035)], VDR [FFBB; 0.2(0.6-0.6] and TNF-¦Á [CCCCGGGGGG; 0.49(0.25-0.97)] were statistically different in patients and control subjects (p<0.05). This study confirmed the association of SNPs in BsmI (B/b + b/b) of VDR and SNPs in -308A (G/A +G/G) of TNF-¦Á genes with susceptibility to tuberculosis in Iranian PTB patients. Furthermore, the extended haplotypes and diplotypes analysis can be considered as an alternative way to determine the host susceptibility to TB.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Tuberculose Pulmonar / Fator de Necrose Tumoral alfa / Receptores de Calcitriol / Predisposição Genética para Doença / Proteínas de Transporte de Cátions Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Humans País/Região como assunto: Asia Idioma: En Revista: Braz. j. infect. dis Assunto da revista: DOENCAS TRANSMISSIVEIS Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Irã / Suécia País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google
Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Tuberculose Pulmonar / Fator de Necrose Tumoral alfa / Receptores de Calcitriol / Predisposição Genética para Doença / Proteínas de Transporte de Cátions Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Humans País/Região como assunto: Asia Idioma: En Revista: Braz. j. infect. dis Assunto da revista: DOENCAS TRANSMISSIVEIS Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Irã / Suécia País de publicação: Brasil