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Comparative evaluation of phenol and thimerosal as preservatives for a candidate vaccine against American cutaneous leishmaniasis
Mayrink, Wilson; Tavares, Carlos Alberto Pereira; Deus, Rosangela Barbosa de; Pinheiro, Melina Barros; Guimarães, Tânia Mara Pinto Dabés; Costa, Carlos Alberto da; Toledo, Vicente de Paulo Coelho Peixoto de.
Afiliação
  • Mayrink, Wilson; s.af
  • Tavares, Carlos Alberto Pereira; Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. BR
  • Deus, Rosangela Barbosa de; Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Farmácia. Ouro Preto. BR
  • Pinheiro, Melina Barros; Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clinicas e Toxicológicas. Belo Horizonte. BR
  • Guimarães, Tânia Mara Pinto Dabés; Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clinicas e Toxicológicas. Belo Horizonte. BR
  • Costa, Carlos Alberto da; Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clinicas e Toxicológicas. Belo Horizonte. BR
  • Toledo, Vicente de Paulo Coelho Peixoto de; Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clinicas e Toxicológicas. Belo Horizonte. BR
Mem. Inst. Oswaldo Cruz ; 105(1): 86-91, Feb. 2010. tab, ilus
Article em En | LILACS | ID: lil-539300
Biblioteca responsável: BR1.1
ABSTRACT
For decades thimerosal has been used as a preservative in the candidate vaccine for cutaneous leishmaniasis, which was developed by Mayrink et al. The use of thimerosal in humans has been banned due to its mercury content. This study addresses the standardization of phenol as a new candidate vaccine preservative. We have found that the proteolytic activity was abolished when the test was conducted using the candidate vaccine added to merthiolate (MtVac) as well as to phenol (PhVac). The Montenegro's skin test conversion rates induced by MtVac and by PhVac was 68.06 percent and 85.9 percent, respectively, and these values were statistically significant (p < 0.05). The proliferative response of peripheral mononuclear blood cells shows that the stimulation index of mice immunized with both candidate vaccines was higher than the one in control animals (p < 0.05). The ability of the candidate vaccines to induce protection in C57BL/10 mice against a challenge with infective Leishmania amazonensis promastigotes was tested and the mice immunized with PhVac developed smaller lesions than the mice immunized with MtVac. Electrophoresis of phenol-preserved antigen revealed a number of proteins, which were better preserved in PhVac. These results do in fact encourage the use of phenol for preserving the immunogenic and biochemical properties of the candidate vaccine for cutaneous leishmaniasis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Conservantes Farmacêuticos / Timerosal / Leishmaniose Cutânea / Fenol / Vacinas contra Leishmaniose Limite: Animals / Female / Humans Idioma: En Revista: Mem. Inst. Oswaldo Cruz Assunto da revista: MEDICINA TROPICAL / PARASITOLOGIA Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google
Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Conservantes Farmacêuticos / Timerosal / Leishmaniose Cutânea / Fenol / Vacinas contra Leishmaniose Limite: Animals / Female / Humans Idioma: En Revista: Mem. Inst. Oswaldo Cruz Assunto da revista: MEDICINA TROPICAL / PARASITOLOGIA Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil