Conversion of barley SNPs into PCR-based markers using dCAPS method
Genet. mol. biol
; Genet. mol. biol;32(3): 564-567, 2009. ilus, tab
Article
em En
| LILACS
| ID: lil-522319
Biblioteca responsável:
BR26.1
ABSTRACT
Molecular genetic research relies heavily on the ability to detect polymorphisms in DNA. Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation in the genome. In combination with a PCR assay, the corresponding SNP can be analyzed as a derived cleaved amplified polymorphic sequence (dCAPS) marker. The dCAPS method exploits the well-known specificity of a restriction endonuclease for its recognition site and can be used to virtually detect any SNP. Here, we describe the use of the dCAPS method for detecting single-nucleotide changes by means of a barley EST, CK569932, PCR-based marker.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Hordeum
/
Polimorfismo de Nucleotídeo Único
Idioma:
En
Revista:
Genet. mol. biol
Assunto da revista:
GENETICA
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Irã
/
Japão
País de publicação:
Brasil