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Analysis of the intercaste transcriptional profile of Melipona scutellaris Latreille, 1811 (Hymenoptera, Apidae, Meliponini) by mRNA differential display
Siquieroli, Ana Carolina S; Vieira, Carlos U; Carvalho-Zilse, Gislene A; Goulart, Luiz R; Kerr, Warwick E; Bonetti, Ana M.
Afiliação
  • Siquieroli, Ana Carolina S; Federal University of Uberlândia. Institute of Genetics and Biochemistry. Uberlândia. BR
  • Vieira, Carlos U; Federal University of Uberlândia. Institute of Genetics and Biochemistry. Uberlândia. BR
  • Carvalho-Zilse, Gislene A; National Institute of Amazon Research. Bee Research Group. Manaus. BR
  • Goulart, Luiz R; Federal University of Uberlândia. Institute of Genetics and Biochemistry. Uberlândia. BR
  • Kerr, Warwick E; Federal University of Uberlândia. Institute of Genetics and Biochemistry. Uberlândia. BR
  • Bonetti, Ana M; Federal University of Uberlândia. Institute of Genetics and Biochemistry. Uberlândia. BR
Biol. Res ; 42(1): 107-110, 2009. ilus, tab
Article em En | LILACS | ID: lil-519089
Biblioteca responsável: BR1.1
ABSTRACT
In colonies of Melipona scutellaris Latreille, 1811 workers can be found with four ganglion nerve cells, a morphological characteristic of the queen. It is hypothesized that these workers, called intercastes, or phenocopies, are phenotypically-like workers, but genotypically identical to queens due to this specific trait. Workers with the same number of ganglion as queens seem to be intercastes between queens and workers. Our objective was to analyze the mRNA pro files of workers, queens, and intercastes of M. scutellaris through DDRT-PCR. Three hundred (300) pupae with white eyes were collected and externally identified according to the number of abdominal nerve ganglions workers (5 ganglions), queens (4 ganglions) and intercastes (4 ganglions). The analysis identified differentially expressed transcripts that were present only in workers, but absent in intercastes and queens, confirming the hypothesis, by demonstrating the environmental effect on the queen genotype that generated phenotype-like workers.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Predomínio Social / Abelhas / RNA Mensageiro / Transcrição Reversa Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Biol. Res Assunto da revista: BIOLOGIA Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Brasil País de publicação: Chile
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google
Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Predomínio Social / Abelhas / RNA Mensageiro / Transcrição Reversa Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Biol. Res Assunto da revista: BIOLOGIA Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Brasil País de publicação: Chile