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Study of genetic divergence among wheat genotypes through random amplified polymorphic DNA
Iqbal, A; Khan, A. S; Khan, I. A; Awan, F. S; Ahmad, A; Khan, A. A.
Afiliação
  • Iqbal, A; University of Agriculture Faisalabad. Centre of Agricultural Biochemistry and Biotechnology. Faisalabad. PK
  • Khan, A. S; University of Agriculture Faisalabad. Centre of Agricultural Biochemistry and Biotechnology. Faisalabad. PK
  • Khan, I. A; University of Agriculture Faisalabad. Centre of Agricultural Biochemistry and Biotechnology. Faisalabad. PK
  • Awan, F. S; University of Agriculture Faisalabad. Centre of Agricultural Biochemistry and Biotechnology. Faisalabad. PK
  • Ahmad, A; University of Agriculture Faisalabad. Centre of Agricultural Biochemistry and Biotechnology. Faisalabad. PK
  • Khan, A. A; University of Agriculture Faisalabad. Centre of Agricultural Biochemistry and Biotechnology. Faisalabad. PK
Genet. mol. res. (Online) ; Genet. mol. res. (Online);6(3): 476-481, 2007. ilus, tab
Article em En | LILACS | ID: lil-498896
Biblioteca responsável: BR26.1
ABSTRACT
The degree of genetic divergence was estimated in seven wheat genotypes, six exotic genotypes and one local variety, through random amplified polymorphic DNA methodology. A total of 112 DNA fragments were generated by the 15 random primers, with an average of about 7.4 bands per primer. Among the 112, 50 fragments showed polymorphism among the seven wheat genotypes. Nei and Li’s similarity matrix ranged from 86.2 to 93.0%, which indicated a narrow genetic base among the genotypes. The maximum similarity, 93.0%, was observed between 12WLRG/1-12 and WL-43. The local variety, Chenab-70, showed the lowest similarity with the exotic types. We conclude that random amplified polymorphic DNA analysis can be used for the characterization and grouping of wheat genotypes; these results will be helpful in our wheat breeding program.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Variação Genética / Triticum / DNA de Plantas / Técnica de Amplificação ao Acaso de DNA Polimórfico Tipo de estudo: Clinical_trials Idioma: En Revista: Genet. mol. res. (Online) Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Paquistão País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Variação Genética / Triticum / DNA de Plantas / Técnica de Amplificação ao Acaso de DNA Polimórfico Tipo de estudo: Clinical_trials Idioma: En Revista: Genet. mol. res. (Online) Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Paquistão País de publicação: Brasil