Characterization of the Arachis (Leguminosae) D genome using fluorescence in situ hybridization (FISH) chromosome markers and total genome DNA hybridization
Genet. mol. biol
; Genet. mol. biol;31(3): 717-724, 2008. ilus, tab
Article
em En
| LILACS
| ID: lil-490061
Biblioteca responsável:
BR1.1
ABSTRACT
Chromosome markers were developed for Arachis glandulifera using fluorescence in situ hybridization (FISH) of the 5S and 45S rRNA genes and heterochromatic 4'-6-diamidino-2-phenylindole (DAPI) positive bands. We used chromosome landmarks identified by these markers to construct the first Arachis species ideogram in which all the homologous chromosomes were precisely identified. The comparison of this ideogram with those published for other Arachis species revealed very poor homeologies with all A and B genome taxa, supporting the special genome constitution (D genome) of A. glandulifera. Genomic affinities were further investigated by dot blot hybridization of biotinylated A. glandulifera total DNA to DNA from several Arachis species, the results indicating that the D genome is positioned between the A and B genomes.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Idioma:
En
Revista:
Genet. mol. biol
Assunto da revista:
GENETICA
Ano de publicação:
2008
Tipo de documento:
Article
/
Project document
País de afiliação:
Argentina
País de publicação:
Brasil