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Genetic diversity analysis of Cuban traditional rice (Oryza sativa L) varieties based on microsatellite markers
Alvarez, Alba; Fuentes, Jorge Luis; Puldón, Violeta; Gómez, Pedro Julio; Mora, Leonor; Duque, Miriam C; Gallego, Gerardo; Tohme, Joe M.
Afiliação
  • Alvarez, Alba; Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear. Departamento de Radiobiología. Ciudad de la Habana. CU
  • Fuentes, Jorge Luis; Universidad Industrial de Santander. Facultad de Ciencias. Escuela de Biología. Bucaramanga. CO
  • Puldón, Violeta; Instituto de Investigaciones del Arroz. Departamento de Mejoramiento Genético. La Habana. CU
  • Gómez, Pedro Julio; Instituto de Investigaciones del Arroz. Departamento de Mejoramiento Genético. La Habana. CU
  • Mora, Leonor; Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear. Departamento de Radiobiología. Ciudad de la Habana. CU
  • Duque, Miriam C; Centro Internacional de Agricultura Tropical. Cali. CO
  • Gallego, Gerardo; Centro Internacional de Agricultura Tropical. Cali. CO
  • Tohme, Joe M; Centro Internacional de Agricultura Tropical. Cali. CO
Genet. mol. biol ; Genet. mol. biol;30(4): 1109-1117, 2007. ilus, tab
Article em En | LILACS | ID: lil-471037
Biblioteca responsável: BR1.1
ABSTRACT
Microsatellite polymorphism was studied in a sample of 39 traditional rice (Oryza sativa L.) varieties and 11 improved varieties widely planted in Cuba. The study was aimed at assessing the extent of genetic variation in traditional and improved varieties and to establish their genetic relationship for breeding purposes. Heterozygosity was analyzed at each microsatellite loci and for each genotype using 10 microsatellite primer pairs. Between varieties genetic relationship was estimated. The number of alleles per microsatellite loci was 4 to 8, averaging 6.6 alleles per locus. Higher heterozygosity (H) was found in traditional varieties (H TV = 0.72) than in improved varieties (H IV = 0.42), and 68 percent of the total microsatellite alleles were found exclusively in the traditional varieties. Genetic diversity, represented by cluster analysis, indicated three different genetic groups based on their origin. Genetic relationship estimates based on the proportion of microsatellite loci with shared alleles indicated that the majority of traditional varieties were poorly related to the improved varieties. We also discuss the more efficient use of the available genetic diversity in future programs involving genetic crosses.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS País/Região como assunto: Cuba Idioma: En Revista: Genet. mol. biol Assunto da revista: GENETICA Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Colômbia / Cuba País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS País/Região como assunto: Cuba Idioma: En Revista: Genet. mol. biol Assunto da revista: GENETICA Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Colômbia / Cuba País de publicação: Brasil